Composite
RQE Lambda

Part:BBa_I744204:Design

Designed by: Thomas Segall-Shapiro   Group: iGEM07_Rice   (2007-07-18)


Tc sensor and cl lambda generator (Tet promoter)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The promoter driving tetR production is crucial to the sensor's sensitivity and range of detection. In this sensor, the tet promoter creates a negative feedback loop, which should keep levels of tetR reasonably low even with high copy number plasmids. Therefore this circuit should remain relatively sensitive to tet at many different copy numbers.

Source

Composite - all parts are drawn from the registry

References